Methylene Blue
$64.99
Comprehensive PubChem-Based Research Overview
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Molecular Identity & Structure
Methylene Blue is recorded in PubChem under CID 6099, with molecular formula C16H18ClN3S and molecular weight ~319.85 g/mol [1]. It is classified as a thiazine dye, existing in cationic form with chloride as a counterion. The liquid format delivers it as a ready-to-use aqueous solution for laboratory protocols.
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Redox Biology & Mitochondrial Research
PubChem annotations describe Methylene Blue as a redox-active compound capable of acting as an electron carrier [1][2]. In vitro studies use it to probe mitochondrial respiration, ROS dynamics, and electron transport chain modulation. Researchers employ it in assays quantifying redox cycling, NADH/NADPH oxidation, and oxidative stress models.
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Cellular Staining & Microscopy
Historically, Methylene Blue has been applied as a biological stain for visualizing cellular structures [1]. In research, the liquid format is used in histological preparations, microbial staining, and cell viability assays, aiding identification of cellular morphology under light microscopy.
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Photodynamic & Reactive Oxygen Studies
PubChem cross-references indicate Methylene Blue’s role as a photosensitizer in controlled photodynamic research [2]. When illuminated, it generates reactive oxygen species (ROS), making it useful in photochemistry experiments, oxidative stress assays, and redox signaling studies.
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Data Traceability & QC
PubChem provides registry identifiers (CAS 61-73-4), spectral data, and depositor-supplied analytical references [1]. This ensures traceability and reproducibility when incorporating Methylene Blue liquid into QC protocols, screening libraries, or redox biology workflows.
Summary
Methylene Blue (CID: 6099) is a heteroaromatic thiazine dye widely documented in PubChem. Supplied as a ready-to-use liquid solution, it is employed in research investigating mitochondrial redox pathways, oxidative stress, and cellular staining. All applications are restricted to research use only. [1][2]
Referenced Citations:
- PubChem Compound Summary — Methylene Blue (CID: 6099)
https://pubchem.ncbi.nlm.nih.gov/compound/6099 - PubChem Substance Record — Methylene Blue
https://pubchem.ncbi.nlm.nih.gov/substance/329756450
Product Usage:
This product is meant only for research use. It is made for lab testing and in vitro experiments (outside a living body), not for use in people or animals. All information on this website is for learning purposes only. Bodily introduction of any kind into humans or animals is strictly forbidden by law. It should only be used by trained professionals in a proper lab setting. This product is not a medicine, food, or cosmetic, and must not be sold or used as one.
Description
Methylene Blue Liquid (PubChem CID: 6099) is a high-purity aqueous solution of the heteroaromatic dye C₁₆H₁₈ClN₃S, supplied for controlled laboratory studies. Commonly used in research on mitochondrial function, redox biology, and cellular staining, this liquid format provides convenience for in vitro assays. For research purposes only.
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